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hekn epidermal skin keratinocytes  (ATCC)


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    ATCC hekn epidermal skin keratinocytes
    Hekn Epidermal Skin Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+skin+keratinocytes/Primary+Epidermal+Keratinocytes%3B+Normal%2C+Human%2C+Neonatal+Foreskin/pm42276073-407-0-4
    Average 99 stars, based on 389 article reviews
    hekn epidermal skin keratinocytes - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Short bio-active peptides for promoting wound healing
    Article Snippet: .. Normal human skin keratinocytes (ATCC CRL-2404) were grown in serum-free keratinocyte growth media supplemented with 5 ng/ml human recombinant epithelial growth factor (EGF) (Life Technologies Corporation, Grand Island, N.Y.). ..

    Article Title: Tetrapeptides derived from human C-X-C chemokines useful for treatment of various skin conditions
    Article Snippet: .. Normal human skin keratinocytes (ATCC CRL-2404) were grown in serum-free keratinocyte growth media supplemented with 5 ng/ml human recombinant epithelial growth factor (EGF) (Life Technologies, Grand Island, N.Y.). ..

    Concentration Assay:

    Article Title: A humanized stromal bed is required for engraftment of isolated human primary squamous cell carcinoma cells in immunocompromised mice
    Article Snippet: Immunofluorescence was performed using standard techniques with the following primary antibodies: pancytokeratin (Dako, clone AE1/3), Mouse MHC class I (AbD Serotec, clone 2G5), Human HLA (AbD Serotec, clone YTH 862.2) and mutant p53 (Calbiochem, clone Pab240). .. For cytospin analysis, normal human skin keratinocytes, RS4 lymphocyte cells (ATCC) or SCCa single cells were washed and resuspended in PBS at a concentration of 10 5 cells per ml. .. 100 μl aliquots of cells were added to each slide cytofunnel (Shandon, Thermo Scientific), spun at 800 rpm for 5 minutes in a cytocentrifuge (Shandon Cytospin 2 cytocentrifuge), fixed in acetone and labelled with a pancytokeratin antibody as above.



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    FIGURE 5 Cytotoxic UVB effect on the HaCaT <t>keratinocyte</t> cell line. (A) The percentage of surviving cells decreased significantly after UVB irradiation was applied from 360 to 5760 mJ/cm2, MTT reduction (***p < 0.0001 vs. 0, one-way ANOVA with Tukey's test). (B) The LD50 was 1247 mJ/cm2 according to the log (inhibitor) versus response, variable slope (four parameters) statistical test. (C) The normality of the data was evaluated using a normal QQ plot: normality and lognormality test (alpha = 0.05).
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    FIGURE 5 Cytotoxic UVB effect on the HaCaT keratinocyte cell line. (A) The percentage of surviving cells decreased significantly after UVB irradiation was applied from 360 to 5760 mJ/cm2, MTT reduction (***p < 0.0001 vs. 0, one-way ANOVA with Tukey's test). (B) The LD50 was 1247 mJ/cm2 according to the log (inhibitor) versus response, variable slope (four parameters) statistical test. (C) The normality of the data was evaluated using a normal QQ plot: normality and lognormality test (alpha = 0.05).

    Journal: Photochemistry and photobiology

    Article Title: Full-spectrum cannabidiol reduces UVB damage through the inhibition of TGF-β1 and the NLRP3 inflammasome.

    doi: 10.1111/php.13993

    Figure Lengend Snippet: FIGURE 5 Cytotoxic UVB effect on the HaCaT keratinocyte cell line. (A) The percentage of surviving cells decreased significantly after UVB irradiation was applied from 360 to 5760 mJ/cm2, MTT reduction (***p < 0.0001 vs. 0, one-way ANOVA with Tukey's test). (B) The LD50 was 1247 mJ/cm2 according to the log (inhibitor) versus response, variable slope (four parameters) statistical test. (C) The normality of the data was evaluated using a normal QQ plot: normality and lognormality test (alpha = 0.05).

    Article Snippet: Human skin- derived keratinocytes (HaCaT cells; American Type Culture Collection (ATCC PCS200- 011) Manassas, VA) were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS; Gibco) and a 1% antibiotic–antimycotic solution (Gibco).

    Techniques: Irradiation

    FIGURE 7 Photoprotective effect of FS-CBD on UVB irradiation-induced damage in the HaCaT keratinocyte cell line. The percentage of viable cells was evaluated after exposure to 0, 1.5, and 3 μg/mL FS-CBD and irradiation with UVB (1080, 1620, and 2160 mJ/cm2). FS-CBD protected against UVB-induced cell death, and viability was evaluated using MTT assay (&&&p < 0.0001 vs. all groups, ωωωp < 0.0001 vs. the 0, 1080 mJ/cm2 group, ψψψ p < 0.0001 vs. the 0, 1620 mJ/cm2 group, *** p < 0.0001 vs. the 0, 2160 mJ/cm2 group, one-way ANOVA with Tukey's test).

    Journal: Photochemistry and photobiology

    Article Title: Full-spectrum cannabidiol reduces UVB damage through the inhibition of TGF-β1 and the NLRP3 inflammasome.

    doi: 10.1111/php.13993

    Figure Lengend Snippet: FIGURE 7 Photoprotective effect of FS-CBD on UVB irradiation-induced damage in the HaCaT keratinocyte cell line. The percentage of viable cells was evaluated after exposure to 0, 1.5, and 3 μg/mL FS-CBD and irradiation with UVB (1080, 1620, and 2160 mJ/cm2). FS-CBD protected against UVB-induced cell death, and viability was evaluated using MTT assay (&&&p < 0.0001 vs. all groups, ωωωp < 0.0001 vs. the 0, 1080 mJ/cm2 group, ψψψ p < 0.0001 vs. the 0, 1620 mJ/cm2 group, *** p < 0.0001 vs. the 0, 2160 mJ/cm2 group, one-way ANOVA with Tukey's test).

    Article Snippet: Human skin- derived keratinocytes (HaCaT cells; American Type Culture Collection (ATCC PCS200- 011) Manassas, VA) were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS; Gibco) and a 1% antibiotic–antimycotic solution (Gibco).

    Techniques: Irradiation, MTT Assay